Tissues and Biomaterial Research Group-(TBRG)Journal of Bioengineering Research2645-56332120200301Studying the physical behavior of human mesenchymal stem cells on the surface of hydroxyapatite after adding graphene as a reinforcement11010250310.22034/jbr.2019.213164.1018ENHassan NosratiDepartment of Materials Engineering, Tarbiat Modares University, Tehran, Iran0000-0003-1509-9819Rasoul Sarraf-MamooryDepartment of Materials Engineering, Tarbiat Modares University, Tehran, IranDang Quang Svend LeDepartment of Clinical Medicine, Aarhus University, DenmarkMaria Canillas PerezInstituto de ceramica y vidrio, csic, Madrid, SpainCody Eric BungerDepartment of Clinical Medicine, Aarhus University, DenmarkJournal Article20191224Introduction: For cell culture assays, different cells have been used so far, including Mouse osteoblast cells (MC3T3-E1), hFOB cells, fibroblast cell, Osteoblast-like MG63 cells, Human osteoblast cells (HFOB 1.19 SV40 transfected osteoblasts), Blood mononuclear cell (PBMC), mesenchymal stem cells (MSCs).<br /> Objective: In this study, the effect of graphene sheets on the physical behavior of stem cells was investigated.<br /> Material and Methods: The hydrothermal method and spark plasma sintering were used in this study. The analysis performed in the sample includes X-ray diffraction, Scanning Electron Microscope, Raman spectroscopy, and cell culture.<br /> Result: The surface of the samples from the hydrophilic (in pure HA) state is somewhat close to the hydrophobic (in HA/rGO nanocomposites) state. It is clear that the physical behavior of the cells on the surface after graphene addition has changed dramatically.<br /> Conclusion: The product of this research (HA/rGO nanostructured composite) has the potential to be used in medical applications.https://www.journalbe.com/article_102503_baad89f5ddfb4c1ba9758201f22c61bb.pdfTissues and Biomaterial Research Group-(TBRG)Journal of Bioengineering Research2645-56332120200302Effect of metalloproteinase-2 on expression of tissue inhibitor of matrix metalloproteinase-4 in cutaneous wound healing in balb/c mice112210483710.22034/jbr.2020.223176.1020ENFateme MashhadiabbasDepartment of Oral and Maxillofacial Pathology, Dental School, Shahid Beheshti University of Medical Science, Tehran, Iran Dental Research Center, Dental School, Shahid Beheshti University of Medical Science, Tehran ,Iran0000000337891727Mehdi KadivarBiochemistry department, Pasteur institute of Iran,Tehran, Iran.0000000243178138Fateme Mahmoodi AfsahDepartment of Oral and Maxillofacial Pathology, Dental School, Shahid Beheshti University of Medical Science, Tehran, Iran0000000312903066Journal Article20200211Introduction: Wound healing comprised serial phases. Though the insitu factors activate to rehabilitate properly immediately after wounding, it eventuate in scar frequently. Collagenases and their inhibitors have dramatic role in processes which prevents scaring. <br /> Objective: We inject “TIMP-4” in cutaneous wound in BALB/C mice to block MMP-2 and search for consequence.<br /> Methods: MMP-2 antibody was injected in dorsal skin of 6-8 weeks BALB/C mice at the level of scapula, on certain periods (4houres, 3 days, 7 days and 14 days after wounding)we collect tissue specimens from dorsal skin of mice using 3mm punch biopsy. We cloned CDNA of MMP-2 and TIMP-4 ,then investigate the expression of genes (using quantitative real time RT-PCR and IHC). The results were analyzed in Wilcoxon Signed Ranks Test, Kruskal-Wallis and Dunn (SPSS version PASW Statistics 18). <br /> .<br /> Results: Four hours after wounding RNA copies and enzymes of MMP-2 increased with lower inflammation in treated samples, RNA copies and enzyme of TIMP-4 was almost similar in the first stage. Three days after injury RNA copies of MMP-2 and TIMP-4 increased and epithelial reattachment was lower in treated group. TIMP-4 enzymes and inflammation rised in third group, the number of RNA copies of TIMP-4 did not change, but the number of RNA copies of MMP-2 decreased in treated samples. Although forth treated group shows lower RNA copies of TIMP-4, the number of RNA copies of MMP-2 increased.<br /> Conclusion: This study indicates that immunotherapy by MMP-2 antibody can lead to regeneration and scar reduction with lower TIMP-4 enzymes.https://www.journalbe.com/article_104837_e3d7976a6c43e0c632a1a4dc2647dee4.pdfTissues and Biomaterial Research Group-(TBRG)Journal of Bioengineering Research2645-56332120200301A Brief Review of Application of Synthetic Biomaterials233310711810.22034/jbr.2020.107118ENJournal Article20200505