@article { author = {Nemati, Shiva and Masudi, Najmeh Sadat}, title = {Comparative Scale-Up Culture of Human Neural Progenitor Cells Derived from Human Embryonic Stem Cell and Transdifferentiated Human Foreskin Fibroblast under Defined Condition in Stirred Bioreactor}, journal = {Journal of Bioengineering Research}, volume = {1}, number = {1}, pages = {1-15}, year = {2019}, publisher = {Tissues and Biomaterial Research Group-(TBRG)}, issn = {2645-5633}, eissn = {}, doi = {10.22034/jbr.2019.84421}, abstract = {Introduction: Generation of clinical grade human neural progenitor population is one of the great promises in stem cell biology which offers a unique opportunity in cell-based therapeutic product. Based on the large number of cells that needed in cell therapy studies, developing a robust bioprocess for large scale expansion of neural progenitors under defined conditions towards achieving clinical goals is a mandatory part before starting any trials. Objective:  Here, we have developed a robust culture system for large scale expansion of human neural progenitor cells derived from human embryonic stem cells and transdifferentiated human foreskin fibroblast in defined medium. Material and Methods: Initially, a chemically defined medium have been developed for expansion of aforementioned cells under static suspension culture. Then, cells were successfully expanded in dynamic system by optimizing conditions such as medium volume, cell inoculation density, dissociation kinetics, aggregate formation and size growth. Results were assessed by immunofluorescent staining, Real-time PCR and karyotype analysis. Result: Cell aggregates were expanded up to ten passages and fold increase reached to 4.2 over a period of 5 days. Finally, after serial passaging of cells in suspension bioreactor, characterization of them in gene and protein expression levels were done in comparison with adherent expanded cells. Conclusion: The result revealed that both expansion systems had same cellular quality and differentiation potencies. Therefore, we could expect to reach a large number of human neural progenitor cells within a few days and this would be a great promise for cell therapy purposes in neurodegenerative diseases}, keywords = {Human neural progenitor cells,Scale up culture,Stirred suspension bioreactor,Chemically define medium}, url = {https://www.journalbe.com/article_84421.html}, eprint = {https://www.journalbe.com/article_84421_16d3970659bad97a82eaacd8ddbff252.pdf} }