Document Type : Original Article
Department of Nanoengineering, Joint School of Nanoscience and Nanoengineering, North Carolina A&T State University, Greensboro, NC, 27401, USA
Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Skin Research Centre, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Charges in liposome composition plays a critical role, so it has significant outcome on the manner of liposome in vitro and in vivo. Neutral and cationic liposomes (LPFS and CLPFS) were prepared and characteristics of LPFS and CLPFS were studied after PHMB encapsulation. According to results, CLPFS exhibited higher cytotoxicity activity, while kept similar size distribution, encapsulation efficiency, lower prolonged retention release profile in comparison to LPFS. CLFPS represented more stable release due to its mutual repulsive force. MTT assay of the produced formulation was examined versus normal primary human skin fibroblast cells and free PHMB presented stronger cytotoxic activity when compared to both cationic and neutral liposomes. It was concluded that PHMB are more toxic to the body but encapsulation liposome specially in neutral liposome can reduce the toxic effect of them.