An Antibacterial Activity effect of a Novel AB Block Copolymer

Document Type : Original Article


1 Institute of Polymeric Materials and Faculty of Polymer Engineering, Sahand University of Technology, Tabriz, Iran

2 Drug Applied Research Center, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran



Various approaches are being developed for the explore of novel and powerful antimicrobial agents, in the form of synthetic polymeric.
Novel poly(2-hydroxyethyl methacrylate)-b-[(N-4-vinylbenzyl),N,N-diethylamine) PHEMA-b-PVEA diblock copolymer was prepared via reversible addition fragmentation transfer (RAFT) polymerization to investigate antibacterial behavior. The structure of the AB diblock copolymer was investigated by means of Fourier transform infrared (FTIR), and 1H nuclear magnetic resonance (NMR) spectroscopies. The molecular weights of PHEMA and PHEMA-b-PVEA segments were calculated to be 10300 and 24000 gmol-1 by GPC, respectively. Furthermore, the antibacterial activity was verified by selecting four types of antibacteria subsuming Staphylococcus aureus (S. aureus), Bacillus cereus (B. cereus), Candidaalbicans (C. albicans) and Escherichia coli (E. coli) as Gram-positive and Gram-negative bacteria models. Results exhibited remarkable fine antibacterial activity. High antibacterial activity effects were observed for C. albicans with PHEMA-b-PVEA diblock copolymers having 44, 75, and 90 mm diameter halo of bacterial inhibition. PHEMA-b-PVEA copolymers could be considered in nanoparticles and antibacterial applications due to their excellent behavior.

Graphical Abstract

An Antibacterial Activity effect of a Novel AB Block Copolymer


[1] Judzewitsch P. R., Nguyen T. K., Shanmugam S., Wong E.H., Boyer C., Towards Sequence‐Controlled Antimicrobial Polymers: Effect of Polymer Block Order on Antimicrobial Activity. Angewandte Chemie 20018; 130: 4649-4654.
[2]. Yang G., Yin H., Liu W., Yang Y., Zou Q., Luo, L., and Li H., Synergistic Ag/TiO2-N photocatalytic system and its enhanced antibacterial activity towards Acinetobacter baumannii. Applied Catalysis B: Environmental 20018; 224: 175-182.
[3]. Shahid S., Khan S. A., Ahmad W., Fatima U., Knawal S., Size-dependent bacterial growth inhibition and antibacterial activity of Ag-doped ZnO nanoparticles under different atmospheric conditions. Indian Journal of Pharmaceutical Sciences 2018; 80: 173-180.
[4]. Nejad Z. D., Jung M. C., & Kim K. H., Remediation of soils contaminated with heavy metals with an emphasis on immobilization technology. Environmental geochemistry and health 2018; 40: 927-953.
[5]. Gong Y., Zhao D., Wang Q., An overview of field-scale studies on remediation of soil contaminated with heavy metals and metalloids: Technical progress over the last decade. Water research 2018.
[6]. Namivandi-Zangeneh R., Kwan R. J., Nguyen T. K., Yeow J., Byrne F. L., Oehlers S. H., Boyer C., The effects of polymer topology and chain length on the antimicrobial activity and hemocompatibility of amphiphilic ternary copolymers. Polymer Chemistry 2018; 9: 1735-1744.
[7]. Zhou X., He J., Zhou C., Strategies from nature: polycaprolactone-based mimetic antimicrobial peptide block copolymers with low cytotoxicity and excellent antibacterial efficiency. Polymer Chemistry 2019; 10: 945-953.
[8]. Lam S. J., Wong E. H., Boyer C., Qiao G. G., Antimicrobial polymeric nanoparticles. Progress in polymer science 2018; 76: 40-64.
[9]. Inam M., Foster J. C., Gao J., Hong Y., Du J., Dove A. P., O'Reilly R. K., Size and shape affects the antimicrobial activity of quaternized nanoparticles. Journal of Polymer Science Part A: Polymer Chemistry 2019; 57: 255-259.
[10] Qiu J.H, Zhang Y.W, Zhang YT, Zhang H.Q, Liu J.D., Synthesis and antibacterial activity of copper-immobilized membrane comprising grafted poly (4-vinylpyridine) chains. Journal of colloid and interface science. 2011; 35:152-9.
[11] Sahiner N., Yasar AO., The generation of desired functional groups on poly (4-vinyl pyridine) particles by post-modification technique for antimicrobial and environmental applications. Journal of colloid and interface science 2013; 15: 402:327-33.
[12] Kavitha T., Kang I.K., Park SY., Poly (4‐vinyl pyridine)‐grafted graphene oxide for drug delivery and antimicrobial applications. Polymer International 2015; 64: 1660-6.
[13] Patel M., Patel R., Chi W.S., Kim J.H., Sung J.S., Antibacterial behaviour of quaternized poly (vinyl chloride)-g-poly (4-vinyl pyridine) graft copolymers. Chinese Journal of Polymer Science 2015; 33: 265-74.
[14] Hernández-Orta M., Pérez E., Cruz-Barba L.E., Sánchez-Castillo M.A., Synthesis of bactericidal polymer coatings by sequential plasma-induced polymerization of 4-vinyl pyridine and gas-phase quaternization of poly-4-vinyl pyridine. Journal of materials science 2018; 53: 8766-85.
[15]. Zhu M., Hua D., Pan H., Wang F., Manshian B., Soenen S. J., Huang C., Green electrospun and crosslinked poly (vinyl alcohol)/poly (acrylic acid) composite membranes for antibacterial effective air filtration. Journal of colloid and interface science 2018; 511: 411-423.
[16]. Cui H., Bai M., Lin L., Plasma-treated poly (ethylene oxide) nanofibers containing tea tree oil/beta-cyclodextrin inclusion complex for antibacterial packaging. Carbohydrate polymers 2018; 179: 360-369.
[17]. Gan D., Xu T., Xing W., Ge X., Fang L., Wang K., Ren F., Lu X., Mussel‐Inspired Contact‐Active Antibacterial Hydrogel with High Cell Affinity, Toughness, and Recoverability. Advanced Functional Materials 2019; 29: 1805964.
[18]. Chen Y., Wilbon P.A., Chen Y. P., Zhou J., Nagarkatti M., Wang C., Chu F., Decho A.W., Tang C., Amphipathic antibacterial agents using cationic methacrylic polymers with natural rosin as pendant group. RSC Advances 2012; 2: 10275-10282.
[19]. Massoumi B., Mousavi-Hamamlu S. V., Ghamkhari A., Jaymand M. A novel strategy for synthesis of polystyrene/Fe3O4 nanocomposite: RAFT polymerization, functionalization, and coordination techniques. Polymer-Plastics Technology and Engineering 2017; 56: 873-882.
[20]. Ghamkhari A., Massoumi B., Salehi R., A new style for synthesis of thermo-responsive Fe3O4/poly (methylmethacrylate-b-N-isopropylacrylamide-b-acrylic acid) magnetic composite nanosphere and theranostic applications. Journal of Biomaterials science Polymer edition 2017; 28: 1985-2005.
[21]. Ghamkhari A., Agbolaghi S., Poorgholy N., Massoumi B., pH-responsive magnetic nanocomposites based on poly (2-succinyloxyethyl methacrylate-co-methylmethacrylate) for anticancer doxorubicin delivery applications. Journal of Polymer Research 2018; 25: 37.
 [22] J. Patel, F. Cockerill, J. Alder, P. Bradford, G. Eliopoulos, D. Hardy, Clinical Laboratory Standards Institute (CLSI.), 2014, 34.
[23] Samadi-Kafil H.,  Mobarez A. M., Journal of King Saud University–Science 2015; 27: 312–317.
[24]. Judzewitsch P. R., Nguyen T. K., Shanmugam S., Wong E. H., Boyer C., Towards Sequence‐Controlled Antimicrobial Polymers: Effect of Polymer Block Order on Antimicrobial Activity. Angewandte Chemie 2018; 130: 4649-4654.
[25]       Ahani E, Montazer M, Toliyat T, Mahmoudi Rad M. A novel biocompatible antibacterial product: Nanoliposomes loaded with poly(hexamethylene biguanide chloride). Journal of Bioactive and Compatible Polymers. 2017;32(3):242-262.
[26]       Ahani E, Montazer M, Toliyat T, Mahmoudi Rad M. Preparation of nano cationic liposome as carriermembrane for polyhexamethylene biguanide chloridethrough various methods utilizing higher antibacterialactivities with low cell toxicity. Journal of microencapsulation.2017;34(2);121-131.